[1]汪灵芝,黄焕森,伍志坤,等.急性切口痛对大鼠脊髓背角缝隙连接蛋白Cx43表达的影响[J].南方医科大学学报,2015,(03):387.
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急性切口痛对大鼠脊髓背角缝隙连接蛋白Cx43表达的影响()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2015年03期
页码:
387
栏目:
出版日期:
2015-03-20

文章信息/Info

Title:
Expression of connexin 43 in spinal cord dorsal horn of rats with acute incisional pain
作者:
汪灵芝;?黄焕森;?伍志坤;?夏正远;?廖敏
关键词:
切口痛;?缝隙连接;?缝隙连接蛋白
Keywords:
incision pain;? gap junction;? connexin
摘要:
目的观察急性切口痛对大鼠脊髓背角缝隙连接蛋白Connexin43(Cx43)表达的影响。方法雄性SD大鼠80只,随机分为
对照组和急性切口痛模型组,切口痛模型组所有大鼠于左后爪作一纵形切口制备切口痛模型,于术前和术后1、2、4、6、24 h时采
用von frey 细丝法测定大鼠左后足机械回缩阈值的改变。于术前和术后2 h、4 h取大鼠左侧脊髓背角组织,分别采用Western
blot 法和免疫荧光标记法检测脊髓背角Cx43蛋白表达的变化。结果急性切口痛组大鼠50%机械性回缩阈值在术后24 h内显
着降低,并于2 h, 4 h 最为显着,与对照组比较差异均有统计学意义(P<0.01);Western blot 结果显示,急性切口痛组脊髓背角
Cx43的表达于术后2 h和4 h明显升高,与对照组比较差异有统计学意义(P<0.01);免疫荧光结果显示,急性切口痛组脊髓背角
Cx43的免疫荧光强度于术后2、4、24 h明显升高,与对照组比较差异有统计学意义(P<0.01)。结论急性切口痛能够引起脊髓
背角缝隙连接蛋白Cx43的表达明显升高,缝隙连接蛋白Cx43可能参与急性切口痛的产生。
Abstract:
Objective To examine the effect of acute incisional pain on the expression of connexin 43 in rat spinal cord dorsal
horn. Methods Eighty rats were assigned into control group without any treatment and incisional pain group with incision
surgery. For paw incisions, a 1-cm longitudinal incision was made through the skin and fascia of the plantar aspect of the right
hind paw. After surgery, the 50% paw withdrawal threshold (PWT) was assessed in response to a tactile stimulus with
calibrated von Frey monofilaments at 1, 2, 4 and 24 h, respectively. The spinal cord dorsal horn of rats was isolated at 1, 2, and
4 h after the surgery to assess the expression of connexin 43 using Western blotting and immunofluorescence assay. Results
The 50% PWT of the rats was significantly decreased after the incision surgery, and this decrement was the most obvious at 2
and 4 h. Western blotting and immunofluorescence assay showed that the expression of connexin 43 in the spinal cord dorsal
horn was significantly increased in rats receiving the surgery especially at 2 and 4 h after the surgery. Conclusion Incision
surgery induces an significant increase in connexin 43 expression in rat spinal cord dorsal horn, suggestting an potential role
of connexin43 in postoperative incisional pain.

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更新日期/Last Update: 1900-01-01